Part:BBa_K346086

RBS(BBa_B0034)+MerT

This part is constructed for the expression of merT, which is a transmembrane protein in E.coli and can transport Hg(II) into the cytosol. MerT is also a member of the Mer operon. MerT is located in the outer membrane of E.coli and have four helix that across the membrane. It use cysteine pair to transfer Hg(II) from merP to the cytosal.

We constructed this part to increase the amount of Hg(II) that can be uptaked into the cell. Since the more Hg(II) in the cell, the more merR dimer will bind Hg(II)and be activated. Those active merR dimer will cause the gene expression under pmerT. This part can thus increase the amount of expression of genes under pmerT by increase cellular Hg(II) concentration.

Figure 1: The role of merT in the transport of Hg(II). This figure indicate the location of merT and the interaction between merP and merT.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 55
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 48